|
KMID : 0043320070300030361
|
|
Archives of Pharmacal Research
2007 Volume.30 No. 3 p.361 ~ p.365
|
|
|
Isolation of Fatty Acids with Anticancer Activity from Protaetia brevitarsis Larva
|
|
Yoo Young-Choon
Shin Byung-Hoon
Hong Jang-Hee
Lee Jung-Lim
Chee Hee-Youn
Song Kyung-Sik
Lee Kyung-Bok
|
|
|
Abstract
|
|
|
|
In this study, biologically active compounds were isolated from Protaetia brevitarsis larva (PBL) by dichloromethane extraction. The dichloromethane extract from PBL was highly cytotoxic to various cancer cells. From a silica gel column chromatograpy of this extract, we obtained four fractions (F-2, F-4, F-5 and F-7) having apoptosis-inducing activity. These fractions induced DNA ladder and caspase-3 activation during apoptosis in colon 26 tumor cells. In 1H and 13C NMR and mass spectral analysis of the fraction F-2 showing the highest apoptosis-inducing activity, we found that the fraction was composed of three free fatty acids such as palmitic acid, (Z)-9-octadecenoic acid and octadecenoic acid. These results indicate that the dichloromethane extract of PBL includes anticancer components composed of at least three fatty acids, and apoptosis-inducing activity of the extract was mediated by caspase-3 activation in tumor cells.
|
|
|
KEYWORD
|
|
|
Protaetia brevitarsis, Apoptosis, Caspase-3, Fatty acid
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|